Methylene Blue Reconstitution Calculator
Reconstitute a 50 mg vial of Methylene Blue with 2.5 mL of bacteriostatic water for a concentration of 20.00 mg/mL — meaning one unit on a U-100 insulin syringe (0.01 mL) holds 200 mcg. Adjust the inputs below for your own vial and dose.
MEDICAL DISCLAIMER: Educational research guidelines only. Lyophilized peptides are investigational chemical compounds and are NOT approved for human consumption, diagnosis, or therapy. Consult a licensed physician before any research application.
Methylene Blue mg-to-units quick reference
At the default 20.00 mg/mL (50 mg in 2.5 mL), draw the following units on a U-100 insulin syringe:
| Target dose | Volume | U-100 units |
|---|---|---|
| 250 mcg (0.25 mg) | 0.013 mL | 1.3 units |
| 500 mcg (0.50 mg) | 0.025 mL | 2.5 units |
| 1000 mcg (1.00 mg) | 0.050 mL | 5.0 units |
Frequently Asked Questions
How do I reconstitute Methylene Blue?expand_more
Add 2.5 mL of bacteriostatic water to the 50 mg Methylene Blue vial for a concentration of 20.00 mg/mL. Inject the water slowly down the vial wall and swirl gently — do not shake.
How many units is a Methylene Blue dose on an insulin syringe?expand_more
At 20.00 mg/mL, one unit on a U-100 syringe (0.01 mL) holds 200 mcg. Divide your target dose in mcg by 200 to get the number of units to draw.
What is the typical Methylene Blue research dose?expand_more
Methylene Blue is typically dosed at 0.5-4 mg/kg orally (~5-30 mg/day low-dose nootropic) in research protocols. See the full titration schedule on the protocol page.